The importance of a good laboratory Notebook

Thomas Edison

Contributed by Paolo Di Mascio

A good scientist knows the importance of keeping all experimental records very well organized. Keeping a good laboratory notebook (LNB) is essential at the time of writing papers and reports, but can also save your time when repetitions are necessary some time later. In fact, this is a practice adopted in various industries where, by legislation, the laboratory procedure must be well documented.

LNBs are, also, important as legal documents to prove patents and defend your data against accusations of fraud. LNB is a Scientific Legacy in the laboratory of your Institution.

Currently, you can also keep an electronic LNB, but in those

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Is cholesterol bad for mitochondria?

Cholesterol

by Sayuri Miyamoto

Cholesterol is an important component of cell membranes and plays essential structural and signaling roles. It is synthesized in the endoplasmic reticulum and distributed to other cell membranes/compartments through a tightly regulated trafficking system involving vesicular and non-vesicular processes [1]. Cholesterol distribution among intra-cellular membranes is not homogeneous. Mitochondria are cholesterol-poor organelles (less than 5 %). However, mitochondrial cholesterol is increased in cancer cell lines and treatment of these cells with statins (cholesterol lowering drugs) increases their susceptibility to chemotherapy [2].

How mitochondrial cholesterol could influence

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More Tequila, please…: Organic nitrogen acquisition in Agave tequilana from endophytic bacteria by oxidative nitrogen scavenging mechanism

Redoxoma Highlights:
 • Superoxide and nitric oxide induced nitrosative processes may follow unusual and different dynamic behaviors
 • Visible, not only UV, light may also damage your hair and skin
 • More Tequila, please…: Organic nitrogen acquisition in Agave tequilana from endophytic bacteria by oxidative nitrogen scavenging mechanism
 • A novel oxidative pathway for human SOD1 aggregation is revealed and may contribute to ALS pathology
 • A novel ubiquitin-related marker in oxidative stress response

by Paolo Di Mascio

Historically, it was thought that plants derived all nitrogen (N) nutrition from the inorganic forms of N, NO3– and NH4+. However, it is now known that the principal form of N entering soils that do not receive inorganic fertilizer is organic N derived from microbial breakdown of organic matter, including amino acids, di and tri-peptides, DNA and proteins. Plants form symbiotic associations with endophytic bacteria

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